A Bright Surprise: Live‐Cell Labeling with Negatively Charged Fluorescent Probes based on Disulfonated Rhodamines and HaloTag
نویسندگان
چکیده
Disulfonated rhodamines are photostable and bright dyes widely used in life science optical microscopy. However, di-sulfonated were considered cell impermeable not applicable living cells. We challenged this assumption with 5 most popular (Rho) having two carboxylic acid residues, versatile sulfonation patterns emitting green (AS488), yellow (Rho530), orange (Rho565) red (Rho590 STAR RED) light. The probes comprising one rhodamine entity a HaloTagTM amine (O2) ligand (x) prepared applied for labeling of living, Vimentin-Halo (VIM-Halo) expressing U-2 OS Surprisingly, we observed specific staining simplest compounds Rho590-x, Rho565-x Rho530-x bearing negative charges; they performed well also stimulated emission depletion (STED) Specific shifts absorption bands other charge; by native chemical ligation esterification.
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ژورنال
عنوان ژورنال: Chemistry methods
سال: 2023
ISSN: ['2628-9725']
DOI: https://doi.org/10.1002/cmtd.202200076